Biohazard Level 2 Pathogens
Actinetobacter calceticus Bacteria
Actinobacillus sp. Bacteria
Actinomyces sp. Bacteria
Adenoviruses Virus
Aeromaonas sp. Bacteria
Aleutian Disease Virus Virus
Anaplasma sp. Parasite
Arachnida propionica Bacteria
Arboviruses Virus BSL 2/3/4
Ascaris sp. Parasite
Avian Erthyroblastosis Virus Virus
Avian Leucosis Virus Virus
Avian Lymphomatosis Virus Virus
Avian Myeloblasotosos Virus Virus
Bacillus alvei Bacteria
Bacillus anthracis Bacteria Tier 1
Bacteroides sp. Bacteria
Blastomyces dermatitides Bacteria
Bordetella pertussis Bacteria
Bordetella sp. Bacteria
Borrelia sp. Bacteria
Bovine Encephalomyelitis Virus Virus
Bovine Leukemia Virus Virus
Bovine Respiratory Syncytial Virus Virus
Bovine Rhinotracheitis (IBR) Virus
Burkholderia (formerly Pseudomonas) mallei & pseudomallei Bacteria BSL 2/3 Tier 1
Cache Valley Virus Virus
Camplobacter sp. Bacteria
Campylobacter fetus var. jejuni Bacteria
Candida albicans Bacteria
Canine Distemper Virus Virus
Canine Hepatitis Virus Virus
Caprine Arthritis Virus
Chlamydia pneumoniae Bacteria BSL 2/3
Chlamydia psittaci Bacteria
Clostridium botulinum Bacteria BSL 2/3 Tier 1
Clostridium tetani Bacteria
Coccidia sp. Parasite
Coccidioides immitis Bacteria BSL 2/3 Tier 1
Corynebacterium diphtheriae Bacteria
Corynebacterium equi Bacteria
Corynebacterium haemolyticum Bacteria
Corynebacterium pseudotuberculosis Bacteria
Corynebacterium pysogenes Bacteria
Corynebacterium renale Bacteria
Coxiella burnetii Rickettsia BSL 2/3 Tier 1
Coxsackie A & B Viruses Virus
Cryptococcus neoformans Bacteria
Cryptosporidia sp. Parasite
Cytomegaloviruses Virus
Dengue Virus Virus
Echinococcus Granulosus Parasite
Echovirus Virus
Ehrlichia sp. Parasite
Encephalomyelitis Virus Virus Tier 1
Encephalomyocarditis Virus Virus
Entamoeba sp. Parasite
Enterobacteriaceae all other Bacteria
Enterobius sp. Parasite
Epidemic Diarrhea Infant Mice Virus
Epidermophyton – pathogenic sp. Bacteria
Epstein-Barr Virus Virus
Erysipelothrix rhusiopathiae Bacteria
Escherichia coli Bacteria
Fasciola sp. Parasite
Feline Leukemia Virus Virus
Feline Sarcoma Virus Virus
Flanders Virus Virus
Francisella tularensis Bacteria BSL 2/3 Tier 1
Fusobacterium sp. Bacteria
Giardia sp. Parasite
Gibbon Ape Lymphosarcoma Virus
Haemobartonella sp. Parasite
Haemophilus sp. Bacteria
Hart Park Virus Virus
Hepatitis A Virus; Hepatitis E Virus Virus
Hepatitis B Virus; Hepatitis C Virus; Hepatitis D Virus Virus
Herpesvirus – other Virus
Herpesvirus ateles Virus
Herpesvirus saimir Virus
Histoplasma capsulatum Bacteria BSL 2/3
Hog Cholera Virus Virus
Human Herpesviruses Virus
Human Immunodeficiency Virus Virus BSL 2/3
Human T-Cell Leukemia Virus I & II Virus
Hymenolepsis nana Parasite
Infectious Bronchitis Virus Virus
Influenza Virus Virus
K (Rate) Virus Virus
Klebsiella sp. Bacteria
Lactic Dehydrogenase Elevating Virus
Langat Virus Virus
Laryngotracheitis Virus Virus
Legionella pneumophilia Bacteria BSL 2/3
Leishmania sp. Parasite
Leptospira interrogans all servars Bacteria
Leukocytozoon sp. Parasite
Listeria sp. Bacteria
Low Risk Oncogenic Viruses Virus
Lymphocytic Choriomeningitis Virus Virus BSL 2/3
Measles Virus Virus
Memingopneumonitis Virus Virus
Microsporum – pathogenic sp. Bacteria
Miscellaneous Molds Bacteria
Moraxella sp. Bacteria
Mouse Encephalomyelitis Virus Virus
Mouse Hepatitis Virus Virus
Mouse Leukemia Virus Virus
Mouse Pneumonia Virus Virus
Mumps Virus Virus
Mycobacterium avium Bacteria
Mycobacterium leprae Bacteria
Mycobacterium sp. Bacteria
Mycobacterium tuberculosis Bacteria BSL 2/3
Mycoplasma sp. Bacteria
Myxomatosis Virus Virus
Naegleria sp. Parasite
Neisseria gonorrhoeae Bacteria BSL 2/3
Neisseria gonorrhoreae Bacteria BSL 2/3
Neisseria menegitidis Bacteria BSL 2/3
Newcastle Disease Virus Virus
Newcastle Disease Virus (VVND) Virus
Nocardia sp. Bacteria
Non-Defective Adenovirus 2SV40 HYB Virus
Papilloma Virus Shope Virus
Paracoccidioides brasilienisis Bacteria
Parainfluenza Virus Virus
Pasteurella sp. Bacteria
Plasmodium sp. Parasite
Poliovirus – all types Virus
Polyoma Virus Virus
Poxvirus sp. (not Smallpox) Virus
Pseudomonas testoserone Bacteria
Pseudorabies Virus
Rabies Virus BSL 2/3
Reovirus sp. Virus
Respiratory Syncytial Virus Virus
Rhinovirus sp. Virus
Rickettsia akari Rickettsia BSL 2/3
Rickettsia australis Rickettsia BSL 2/3
Rickettsia canada Rickettsia BSL 2/3
Rickettsia conorii Rickettsia BSL 2/3
Rickettsia prowazekii Rickettsia BSL 2/3 Tier 1
Rickettsia rickettsii Rickettsia BSL 2/3 Tier 1
Rickettsia siberica Rickettsia BSL 2/3
Rickettsia tsutsugamushi Rickettsia BSL 2/3
Rickettsia typhi (R. mooseri) Rickettsia BSL 2/3
Rochalimaea quintana Rickettsia
Rochalimaea vinsonii Rickettsia
Rotococcus (Coryne.) equi Bacteria
Rous Sarcoma Virus Virus
Rubella Virus Virus
Salmonella sp. Bacteria
Salmonella typhi Bacteria BSL 2/3
Sarcocystis sp. Parasite
Schistosoma sp. Parasite
Shigella sp. Bacteria
Simian Immunodeficiency Virus BSL 2/3
Simian T-Cell Leukemia Virus Virus
Simian Virus – other Virus
Sindbis Virus Virus
Slow Viruses Virus
Sporothrix schenckii Bacteria
Spotted Fever Group – other Rickettsia BSL 2/3
Staphylococcus sp. Bacteria
Streptocacillus moniliformis Bacteria
Streptococcus sp. Bacteria
Streptomyces somaliensis Bacteria
Strongyloides sp. Parasite
Taenia solium Parasite
Tensaw Virus Virus
Toxocara canis Parasite
Toxoplasma sp. Parasite
Transmissible Spongiform Encephalopathies (Creutzfeldt-Jakob; kuru; and related agents Virus
Treponema pallidum Bacteria
Trichinella spiralis Parasite
Trichophyton – pathogenic sp. Bacteria
Trypanosoma sp. Parasite
Turlock Virus Virus
Vaccinia Virus Virus
Vesicular Stomatitis Virus – lab adapted Virus
Vibrio sp. Bacteria
Yaba Virus Virus
Yellow Fever Virus 17D Strain Virus Tier 1
Yersinia pestis Bacteria BSL 2/3 Tier 1
Builds on Biohazard Level 1
- BSL-2 builds upon the containment requirements of BSL-1 and is the highest level of biological safety.
Laboratory Practices
- Change clothing before entering.
- Shower upon exiting.
- Decontaminate all materials before exiting.
Safety Equipment
- All work with the microbe must be performed within an appropriate Class III BSC , or by wearing a full body, air-supplied, positive pressure A suit.
Facility Construction
- The laboratory is in a separate building or in an isolated and restricted zone of the building.
- The laboratory has dedicated supply and exhaust air, as well as vacuum lines and decontamination systems
Biosafety Level 2 Laboratory
Book and Video Picks
These videos/books are some that have a lot of educational information about serious viral epidemics.
Facing Darkness
Samaritan's Purse
This is a dramtic documentary how Samaritan's Purse was called on by Medicine without Frontiers, the only other organization in the world to come alongside and fight Ebola in the Liberia.
Dengue. The Hunt for a Vaccine
Journeyman Pictures
This documentary will show what the rest of the world deals with in this painful disease.
Surviving Ebola
NOVA
This is a good general PBS documentary of Ebola.
The Hot Zone
Book & TV Series
The National Geographic made-for-TV series came from the book by Richard Preston. This documents the case of Ebola Reston, which erupted in Reston, Virgina. The TV series takes some artistic liberties, and the book is much more detailed. It is worth experiencing both, however.